Laboratory Supplies

Lee Biosolutions has announced the production and availability of five Human Lactate Dehydrogenase Isoenzymes - LDH-1, LDH-2, LDH-3, LDH-4 and LDH-5 - for purchase at LeeBio.com. Refer to Item No. 355-15 for the most popular LDH Isoenzyme product from the humanheart, LDH-1.

Human lactate dehydrogenase isoenzymes are primarily used by cells in specific tissue. LDH, also known as lactic dehydrogenase, is responsible for converting muscle lactic acid into pyruvic acid, an essential step in producing cellular energy.

When injury occurs, cells containing specific LDH isoenzymes are released in the bloodstream. Analyzing specific LDH isoenzyme ratios helps identify certain patterns in order to aid in the diagnosis ofdisease such as heart attack, lung injury, lung disease, liver or muscle disease, advanced cancers and autoimmune diseases.

According to Lee Biosolutions President Burton Lee, the company keeps close tabs on the clinical research community in an effort to stay ahead of widespread demands for products like Human Lactate Dehydrogenase Isoenzymes.

"Lee Biosolutions has developed a reputation for high-quality biomarker production," said Lee. "We isolate LDH isoenzymes from the humanheart , human red blood cells and the human liver. As a manufacturer of high purity human enzymes, we are able to custom fill orders and we will, likewise, place the protein in the buffer of your choice, if required."

Burton also indicated Lee Biosolutions focuses on providing consistently pure products to the clinical research community. "We continue to expand our product line based on developing trends in the industry," he added.

Lee Biosolutions is a leader in the biomaterials industry, providing quality biomaterial products to clinical labs and researchers around the world, developing tomorrow's cures. The company has provided the proteins used in a number of high profile biomarker studies. For more information or to place an order, contact Lee Biosolutions at 314-968-1091, or visit the company's newly redesigned website, http://www.leebio.com.

EPICENTRE's BACMAX™ DNA Purification Kit enables easy, reliable isolation of exceptionally pure intact BAC DNA without binding columns, resins, or organic extractions. The high-quality BAC DNA is suitable for many applications, including sequencing, fingerprinting, PCR, and preparation of shotgun libraries. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25 µg of BAC DNA from a 1.5 to 100 ml culture of a single-copy BAC. Selective precipitation steps and the incorporation of EPICENTRE's RiboShredder™ RNase Blend remove contaminants that degrade DNA and interfere with downstream applications.

Thermo Fisher Scientific, the world leader in serving science, has introduced a new range of Thermo Scientific Verso™ cDNA synthesis kits and one-step reverse transcription - polymerase chain reaction (RT-PCR) kits. These kits combine a new reverse transcription enzyme, improved priming options and optimised buffers to generate high yield of full length cDNA. Designed to save time by minimising the steps involved in the RT-PCR protocol, the kits allow users to benefit from optimum flexibility and sensitivity in an easy to use format.

The new Verso RT Enzyme utilised in the kits has an improved dynamic range which maximises sensitivity and allows users to detect a wider range of starting template concentrations. The enzyme mix also includes an RNase inhibitor which significantly reduces RNase contamination whilst simplifying pre-RT setup steps.

Verso RT-PCR kits offer laboratories the flexibility of using anchored oligo dT or random hexamers in a reaction. The combination of these primers in the reverse transcription step gives greater coverage and yield of cDNA from RNA, resulting in greater reaction efficiency.

Several kit options are available in the Thermo Scientific Verso range to meet individual users’ specific needs. These include kits incorporating the ReddyMix™ buffer system which save time by allowing PCR products to be loaded directly onto an agarose gel. Kits are also available with Thermo-Start™ DNA polymerase in the master mix for maximum specificity in the PCR reaction.

The Verso RT Enzyme is also included in a new range of Thermo Scientific Verso QRT-PCR kits for QPCR users.polymerase in the master mix for maximum specificity in the PCR reaction.

The Verso RT Enzyme is also included in a new range of Thermo Scientific Verso QRT-PCR kits for QPCR users.

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Traditional approaches to large scale genotyping involve numerous microwell plates, large amounts of costly reagents and dispensing robotics to cope with the intensive liquid handling.

According to Mike Lucero, executive vice president of Sales and Marketing at Fluidigm, the IFCs are three dimensional microfluidic devices that have 48 reagent ports and 48 reaction ports that allow 2,304 parallel nano-volume reactions in each IFC.

This is achieved by driving a precise amount of reagents and reactants into chambers in the IFC that are limited by valves.

"Microfluidics got the reputation for not being able to find the appropriate application - but its inside out because in the past microfluidics didn't have the valves so they couldn't achieve the integration and miniaturisation that we have now - it's the valves that make the difference," said Lucero

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